Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.098324 |
Chromosome: | chromosome 16 |
Location: | 70773 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g695800 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCAAGCGAGTCGATGGTCGACAGCCAAC |
Internal bar code: | GCCTCGTAGGAATCATGTTGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 161 |
LEAP-Seq percent confirming: | 99.0172 |
LEAP-Seq n confirming: | 403 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCCTGACTGACAACACATT |
Suggested primer 2: | GGAGTGTTTTGAGCTCAGGG |