| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098332 |
| Chromosome: | chromosome 4 |
| Location: | 430593 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217921 | COPB1 | Beta subunit of COP-I complex; (1 of 1) K17301 - coatomer, subunit beta (COPB1, SEC26) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCATAATACACACCGCTGCCGCCTTCC |
| Internal bar code: | CTTCGGCGCGGTTCTCAAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 99.6586 |
| LEAP-Seq n confirming: | 2627 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCCCGCTACTCCACAATC |
| Suggested primer 2: | CTCTTTAGGTATGCGGAGCG |