| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098346 |
| Chromosome: | chromosome 10 |
| Location: | 5484101 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458950 | IBA57 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTACGCACCCTGCCATACCCACACACC |
| Internal bar code: | ACGTTCAGTTGGGCGCGGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 31.1491 |
| LEAP-Seq n confirming: | 1003 |
| LEAP-Seq n nonconfirming: | 2217 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGATGGTTAGTGGGTGG |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |