| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.098352 |
| Chromosome: | chromosome 10 |
| Location: | 3073842 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441600 | OPR48 | OctotricoPeptide Repeat protein 48 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGGGAGCAGGTGGGGCCTTGGGTGTT |
| Internal bar code: | GGGGCGTGTGGCTCTTAGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 98.535 |
| LEAP-Seq n confirming: | 12645 |
| LEAP-Seq n nonconfirming: | 188 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGTTTGACGCAGTGAC |
| Suggested primer 2: | ACTGCGTGTTCTTGGCAAC |