| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.098359 |
| Chromosome: | chromosome 3 |
| Location: | 3027828 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164000 | TEF7 | Predicted protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGCATTGTGCTTGCACTGCGCGTCTAA |
| Internal bar code: | CAATTGTACTCTGCAATTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 84.493 |
| LEAP-Seq n confirming: | 425 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGGTCCTCAAGTGGTGT |
| Suggested primer 2: | CCCCGTACTCACAATGACCT |