| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098377 |
| Chromosome: | chromosome 1 |
| Location: | 3348889 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021150 | TRMC2,TMU1 | (1 of 2) 2.1.1.35 - tRNA (uracil(54)-C(5))-methyltransferase / tRNA:m(5)U(54)-methyltransferase; tRNA (uracil-5-)-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGAATGAACTGCCCCCACCCCAACCCC |
| Internal bar code: | CCAGGTTTCAAGTTTAATCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 99.8348 |
| LEAP-Seq n confirming: | 6042 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGAGCCAATCAAGGAC |
| Suggested primer 2: | GATCGTCGGCAAACGTTAAT |