| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.098413 |
| Chromosome: | chromosome 1 |
| Location: | 7827334 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g057061 | (1 of 1) PF08167 - rRNA processing/ribosome biogenesis (RIX1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTGCAGCCGCAGCTGCCGCCGGTGGTC |
| Internal bar code: | GTTCTGTTATGCTGAACCCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 51 |
| LEAP-Seq percent confirming: | 54.8611 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTTGACGGACACGACGAC |
| Suggested primer 2: | GCGTCACACTCCTCTCACAA |