| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098429 |
| Chromosome: | chromosome 4 |
| Location: | 3026230 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225100 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTTGTGGTGGTGGTGTTGCTGCTGCAGG |
| Internal bar code: | GTGGTCATACGCGAGCCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 99.2328 |
| LEAP-Seq n confirming: | 4398 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGATGCCGTCCAAAAAGAG |
| Suggested primer 2: | GAATGAACCAATCCCACCAC |