| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.098483 |
| Chromosome: | chromosome 7 |
| Location: | 4129418 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342800 | CGL16 | (1 of 1) PF07063 - Domain of unknown function (DUF1338) (DUF1338); Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCGTTGCCTGGTGGCCAGCTATGCTTT |
| Internal bar code: | CTATCTGCGAGTGGTCAACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 98.6175 |
| LEAP-Seq n confirming: | 6206 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTTGTCTGGGCTTCGTT |
| Suggested primer 2: | ATGCCAGCATTTGTGTGTGT |