Insertion junction: LMJ.RY0402.098505_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.098505
Chromosome:chromosome 14
Location:1820779
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g620200 (1 of 1) IPR011527//IPR021261 - ABC transporter type 1, transmembrane domain // Protein of unknown function DUF2838 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTGTTGCATTGTCCACTGATTAACGCGC
Internal bar code:GTCCATAAAGATGCGGGGGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:713
LEAP-Seq percent confirming:99.0772
LEAP-Seq n confirming:7408
LEAP-Seq n nonconfirming:69
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTCGTATGTGTGTCCGC
Suggested primer 2:GGTACTAGTGGAGAGCCCCC