| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.098528 |
| Chromosome: | chromosome 1 |
| Location: | 4043652 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026300 | (1 of 3) PF08238 - Sel1 repeat (Sel1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTACTTGGAACGCACTAAACAGACACA |
| Internal bar code: | TTCTTAGTCACGAGTACACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 99.7224 |
| LEAP-Seq n confirming: | 4670 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTCATAAACAGGGCCTC |
| Suggested primer 2: | AACCTAACCCTATCACCCGC |