| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098534 |
| Chromosome: | chromosome 17 |
| Location: | 7029663 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746647 | PUF2 | (1 of 1) PTHR12537:SF12 - MATERNAL PROTEIN PUMILIO; Puf protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAAGGTCACGTACGGTGGTGGCTCGAAC |
| Internal bar code: | GAACCCTGTGTCGCGCGTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 942 |
| LEAP-Seq percent confirming: | 98.3493 |
| LEAP-Seq n confirming: | 6971 |
| LEAP-Seq n nonconfirming: | 117 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACACCTACGGCAAGCACA |
| Suggested primer 2: | CAACGACGTGTATGAATGGC |