| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098572 |
| Chromosome: | chromosome 9 |
| Location: | 1863432 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395650 | (1 of 2) PF13806 - Rieske-like [2Fe-2S] domain (Rieske_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTTGCCCTTGGACAGCAGCTCCGTCTCT |
| Internal bar code: | TGACGGGGGGTTAGGCATTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 98.7228 |
| LEAP-Seq n confirming: | 2087 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGAATACTCACAAGCGGT |
| Suggested primer 2: | TTGATGCAAGCCACAAACAT |