Insertion junction: LMJ.RY0402.098582_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.098582
Chromosome:chromosome 8
Location:254302
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g358554 (1 of 14) 3.1.3.16//3.1.3.48 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Protein-tyrosine-phosphatase / PTPase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACATATTGCTATTACTGTCACTCGAACT
Internal bar code:GACTGCCGCACCATCTCGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:540
LEAP-Seq percent confirming:96.3357
LEAP-Seq n confirming:815
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTGGGAAAGCAAAAGGGT
Suggested primer 2:ATGGTCACCACACAGGTCAA