| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098596 |
| Chromosome: | chromosome 7 |
| Location: | 5148075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTGTGGTCGGACTTGCGGCCCTGCTTG |
| Internal bar code: | TCAGAGACGATGCCGTGTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 98.3023 |
| LEAP-Seq n confirming: | 6022 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCTTTGAGCGGTCTACA |
| Suggested primer 2: | TGGACGTGTTGATCTGGTGT |