| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098600 |
| Chromosome: | chromosome 11 |
| Location: | 1225271 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467698 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGGAGGCATGGTGAGGCGGGCGGGCGT |
| Internal bar code: | ATGCATGGCCCTGGGCTGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 98.8699 |
| LEAP-Seq n confirming: | 3762 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGTGCCTCCAACTCTA |
| Suggested primer 2: | TGGGTGCTCTAGCTCCAACT |