Insertion junction: LMJ.RY0402.098613_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.098613
Chromosome:chromosome 5
Location:1442690
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g244050 Chlamydomonas specific protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAATGGCCTGCTGCTTGCGCCCGCGCTTG
Internal bar code:TCGTACGTGCATATGGTGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:619
LEAP-Seq percent confirming:99.2133
LEAP-Seq n confirming:3531
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AGACGCTCTGACTCCTCTGC
Suggested primer 2:GGCAAAGGTTGAAGCTGAAG