| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098647 |
| Chromosome: | chromosome 10 |
| Location: | 6310058 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465300 | (1 of 89) 3.6.4.13 - RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCGAGCGACGGCGTGTGGCGGTGCACG |
| Internal bar code: | TTCTCGTAGCGGATTGGAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1105 |
| LEAP-Seq percent confirming: | 99.5717 |
| LEAP-Seq n confirming: | 3720 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCTCCAAAGTTCAGAGG |
| Suggested primer 2: | GAAACGCTCTGCTGATACCC |