Insertion junction: LMJ.RY0402.098657_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.098657
Chromosome:chromosome 16
Location:582466
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g692350 (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAGCCAGGTAGGACAGGGGGCGCAGAC
Internal bar code:CTCGAGTCGGGAGGTAGGGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:626
LEAP-Seq percent confirming:98.4674
LEAP-Seq n confirming:3855
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:ATACCAGGCAAAAGACCACG
Suggested primer 2:GAGCTGTGTGCTGCTACTGC