| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.098669 |
| Chromosome: | chromosome_13 |
| Location: | 1497084 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g572650 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCTCGGTCAGGTGGCAACAGCTCCAATTT |
| Internal bar code: | CAAAAACCGGCTAGGTAGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 91 |
| LEAP-Seq percent confirming: | 7.43507 |
| LEAP-Seq n confirming: | 438 |
| LEAP-Seq n nonconfirming: | 5453 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGACCTCAAAACACAACT |
| Suggested primer 2: | CGAGGCAGGCAGTAAGGTAG |