| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098673 |
| Chromosome: | chromosome 3 |
| Location: | 4929032 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179860 | (1 of 1) 2.1.1.203 - tRNA (cytosine(34)-C(5))-methyltransferase; tRNA-(m5C) methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGTGTCAATGCTACTGAGTTAACAGCT |
| Internal bar code: | GACGGGTGTTGCATTGTTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 99.4181 |
| LEAP-Seq n confirming: | 1196 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGAAAAGAGGTCACTCG |
| Suggested primer 2: | TAGTGGAAGGTGGTTGAGGG |