| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098675 |
| Chromosome: | chromosome 12 |
| Location: | 1437069 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487900 | FDX9 | (1 of 13) PF00111 - 2Fe-2S iron-sulfur cluster binding domain (Fer2); Ferredoxin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGTACCGAACGCGCCTGCTGGGACGGGG |
| Internal bar code: | ACCTAGGTTTCGCCTTGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.3117 |
| LEAP-Seq n confirming: | 3030 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTCACCTTGCAGACGCAG |
| Suggested primer 2: | CGTTGAGTGAGGCATTGAGA |