| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.098685 |
| Chromosome: | chromosome 8 |
| Location: | 3331279 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374000 | (1 of 1) PF10444 - Nbl1 / Borealin N terminal (Nbl1_Borealin_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCAATCGTACACTGGGGACCGCACAT |
| Internal bar code: | GATGGCAAGGGCATCTGGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 99.4884 |
| LEAP-Seq n confirming: | 10112 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTTCACAGAGGTGC |
| Suggested primer 2: | CACTAACGAGGTGGTGCAGA |