Insertion junction: LMJ.RY0402.098693_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.098693
Chromosome:chromosome 11
Location:2625145
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g476376 FAP221,Pcdp1,RPC82 Flagellar Associated Protein 221; (1 of 1) PTHR23053//PTHR23053:SF21 - DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTTGCACATGTAACTGTACGACGGCTGG
Internal bar code:CTTAGGCCGCGGTGACCTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:703
LEAP-Seq percent confirming:99.0067
LEAP-Seq n confirming:9768
LEAP-Seq n nonconfirming:98
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CAATTCGCTCTGCACCTGTA
Suggested primer 2:TCAGTGCTGGACTTCGTTTG