| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098758 |
| Chromosome: | chromosome 16 |
| Location: | 6769627 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673841 | (1 of 1) PTHR13481:SF0 - UPF0454 PROTEIN C12ORF49 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCAACTCCCAACGCTCACTTGCAAGGGC |
| Internal bar code: | GAGTGTAACTGCGCGATCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.8423 |
| LEAP-Seq n confirming: | 633 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGGAAACCTTGCTAGAC |
| Suggested primer 2: | CACGGACATTGGCATAACAG |