Insertion junction: LMJ.RY0402.098762_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.098762
Chromosome:chromosome 1
Location:3290817
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g020800 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGTGGCGCAAGGACGCATGGCATCATCC
Internal bar code:GATGGGGTGCGTCAATTTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:424
LEAP-Seq percent confirming:99.5421
LEAP-Seq n confirming:1087
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAGCAGCAGTCAGGGTA
Suggested primer 2:ATGGGTAGTTGCCAACGAAG