| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098767 |
| Chromosome: | chromosome 7 |
| Location: | 1432979 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323050 | PEN2 | Presenilin enhancer | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTATACAAGTACTTGTTTACGATTCAAGC |
| Internal bar code: | ACAACGGACGTTCTAATGATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.1646 |
| LEAP-Seq n confirming: | 2730 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGTGCAGGATGGTGGAT |
| Suggested primer 2: | TGCCTTACAGGGGTAACAGG |