| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098784 |
| Chromosome: | chromosome 10 |
| Location: | 2398038 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435350 | ESU1,ESU1a | Endosulfine cAMP-regulated phosphoprotein; (1 of 2) PF04667 - cAMP-regulated phosphoprotein/endosulfine conserved region (Endosulfine) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGCTAGGATAAAAGCGGCGGGGCGTT |
| Internal bar code: | GCGGGGCAGATTGACGGAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 99.0531 |
| LEAP-Seq n confirming: | 1883 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCGTCTGACGTACGAA |
| Suggested primer 2: | CTAGAGGTAGCGGTGCAAGG |