| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.098822 |
| Chromosome: | chromosome 12 |
| Location: | 2736532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503400 | PRP38 | (1 of 1) K12849 - pre-mRNA-splicing factor 38A (PRPF38A); Pre-mRNA splicing factor, SR-related protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCTGTGCATCCAGGCGCCTATAACTGC |
| Internal bar code: | CAGCCCCAATCCCAGGTGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 752 |
| LEAP-Seq percent confirming: | 97.683 |
| LEAP-Seq n confirming: | 3457 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGGTTGGGAACGCACAAG |
| Suggested primer 2: | TAAGGACAAGCCGAAGGAGA |