| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.098888 |
| Chromosome: | chromosome 3 |
| Location: | 5291040 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183650 | GDP4,GDPD4 | (1 of 3) K01126 - glycerophosphoryl diester phosphodiesterase (E3.1.4.46, glpQ, ugpQ); Glycerophosphoryl diester phosphodiesterase family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACAGTCGCTGTCGGAGATCGCGGGGTG |
| Internal bar code: | CAACGCCGAGTTATTGCCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 209 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATACGGAGACCAAGCACCC |
| Suggested primer 2: | TACTCGTCGGCAACTGTCAC |