| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098919 |
| Chromosome: | chromosome 2 |
| Location: | 5029963 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104400 | (1 of 1) IPR000104//IPR005033 - Antifreeze protein, type I // YEATS | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGCTGCTGGGGCGGGTGGCGCCGGCA |
| Internal bar code: | ACAGGCGTAAACTGCCCGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 99.3504 |
| LEAP-Seq n confirming: | 2753 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTAGGCAGTTGCACAGT |
| Suggested primer 2: | CTGCTGTGAGGTAGACGCAG |