Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.098991 |
Chromosome: | chromosome_8 |
Location: | 981916 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362000 | FAP72 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TATGAGTTAATGGGGTCAGTGTCTACGCAT |
Internal bar code: | GCTTGCAAAAGTTTCCGCACTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 683 |
LEAP-Seq percent confirming: | 75.3735 |
LEAP-Seq n confirming: | 1665 |
LEAP-Seq n nonconfirming: | 544 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGTTCGTTCTCTTCTCCAC |
Suggested primer 2: | GCAGAAGAAGGCGGTGTAAG |