| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099015 |
| Chromosome: | chromosome 16 |
| Location: | 3170344 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666100 | (1 of 1) K14168 - cytoplasmic tRNA 2-thiolation protein 1 (CTU1, NCS6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGACGGCTGGGGAGGACGGCATTCACCT |
| Internal bar code: | TGTAAGTGGTGACCTTCTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 99.9004 |
| LEAP-Seq n confirming: | 3008 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTGCTAACACTACCAT |
| Suggested primer 2: | GTAATCAGGGCGGATCTTCA |