| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099031 |
| Chromosome: | chromosome 16 |
| Location: | 6348777 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGCACCCGATCACCCACAGCCCCCAAG |
| Internal bar code: | CGCGATAACTGCGGTGTGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 98.7805 |
| LEAP-Seq n confirming: | 243 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGACACGAGGATTCGAC |
| Suggested primer 2: | TACGGGATGCTACGACACAC |