| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099036 |
| Chromosome: | chromosome 12 |
| Location: | 2427579 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507051 | (1 of 2) K01193 - beta-fructofuranosidase (E3.2.1.26, sacA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGGCCAGCAGTACCCGTACGACGACCC |
| Internal bar code: | GTCGGCCTGTTGCCTGTCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 98.9865 |
| LEAP-Seq n confirming: | 293 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTATTCCTGCTCCTGCTC |
| Suggested primer 2: | GGTGCTGCTACAAATGCTGA |