| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099037 |
| Chromosome: | chromosome 12 |
| Location: | 8342000 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549450 | (1 of 1) 3.7.1.5 - Acylpyruvate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTGCCGGCCGCGCGACCGACAGCCTGA |
| Internal bar code: | GTGGACAACGGACTAGAACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 73.9558 |
| LEAP-Seq n confirming: | 903 |
| LEAP-Seq n nonconfirming: | 318 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACTTAGAGGCAGCGTTC |
| Suggested primer 2: | AACTGAATGAACTGACCCCG |