Insertion junction: LMJ.RY0402.099049_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.099049
Chromosome:chromosome 12
Location:5541634
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g531350 CGL62 putative cell cycle associated protein; (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGGAGTCAATTTCCTGTGCTATGTACGG
Internal bar code:CGGTTCGGAGATCAGCAACCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:921
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGACACTGCTGTGTGTGTTG
Suggested primer 2:GGTCAACATCGTGGACAGTG