Insertion junction: LMJ.RY0402.099049_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.099049
Chromosome:chromosome 12
Location:5541691
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g531350 CGL62 putative cell cycle associated protein; (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTGTTGGGCAAGTCCCAGAGAATACGGT
Internal bar code:GCTCAGATCGCGCACGTTACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:585
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:578
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCAACATCGTGGACAGTG
Suggested primer 2:CAGTGAACACCAGACGCTGT