| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099061 |
| Chromosome: | chromosome 16 |
| Location: | 2224967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658750 | (1 of 4) PTHR14255//PTHR14255:SF3 - ATP-DEPENDENT PROTEASE CEREBLON // SULFITE EXPORTER TAUE/SAFE FAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCGCCTTCACAGACGAGGCCCCGACG |
| Internal bar code: | TGTCTTACTCCTCTTTGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1139 |
| LEAP-Seq percent confirming: | 98.3333 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCTTGCTTGGTTCTCGT |
| Suggested primer 2: | GCTCAGTATTGGCATGAGCA |