| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099068 |
| Chromosome: | chromosome 6 |
| Location: | 8831917 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310450 | (1 of 1) K03124 - transcription initiation factor TFIIB (TFIIB, GTF2B, SUA7, tfb) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACGAAGACTGCAGGGTGGGACGGACTG |
| Internal bar code: | AGCAGGGTGCGACTCTTGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 99.2058 |
| LEAP-Seq n confirming: | 4247 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTGTACAAGGACCTGTT |
| Suggested primer 2: | AGGGCCGACAACTGTAACAC |