| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099084 |
| Chromosome: | chromosome 9 |
| Location: | 7515765 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414050 | Pontin,RVB1,RUVBL1 | Nucleoside-triphosphatase RuvB-like protein; (1 of 1) K04499 - RuvB-like protein 1 (pontin 52) (RUVBL1, RVB1, INO80H) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCTTGAAGAGGCCAGGCGAGGGAACGG |
| Internal bar code: | GTGCATTGCGGTTACTTGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 99.6765 |
| LEAP-Seq n confirming: | 9860 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTCACAGTGGCTCTCC |
| Suggested primer 2: | AGCTTTCACTTGGGCTTTGA |