| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099100 |
| Chromosome: | chromosome 2 |
| Location: | 3900278 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096200 | (1 of 1) K06990 - uncharacterized protein (K06990) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCTGCTGACAGCCCCCACGGTTGGAAG |
| Internal bar code: | GCCGAGGATATGGAGGGTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 96.9152 |
| LEAP-Seq n confirming: | 754 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACGTTTTGTTGTGTGCC |
| Suggested primer 2: | ACGAAAGCGAAGAAGACCAA |