| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099104 |
| Chromosome: | chromosome 11 |
| Location: | 617556 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467613 | (1 of 1) IPR000742//IPR001881 - EGF-like domain // EGF-like calcium-binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTCACAGAAGACGCCGAGTGCAAGGCC |
| Internal bar code: | AGGATGGATTCCGGGCGCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 98.9223 |
| LEAP-Seq n confirming: | 1744 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAAGGCTTGTCGACTTC |
| Suggested primer 2: | AAATGAAACATCCCCATCCA |