| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099105 |
| Chromosome: | scaffold 26 |
| Location: | 41502 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre26.g756897 | (1 of 1) IPR000104//IPR003903 - Antifreeze protein, type I // Ubiquitin interacting motif | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGACGCACAGCTTCGCGCTGGCTGTT |
| Internal bar code: | CGCTTCCTAAGTCCACCTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 519 |
| LEAP-Seq percent confirming: | 91.4928 |
| LEAP-Seq n confirming: | 570 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTAGCGGCAGTAGCAGTG |
| Suggested primer 2: | GGTGTAACAAAGGGGCTTGA |