Insertion junction: LMJ.RY0402.099120_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.099120
Chromosome:chromosome 7
Location:4643006
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g344350 TPP1 Chloroplast thylakoid processing peptidase; (1 of 1) K03100 - signal peptidase I (lepB) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCAATGTGGCCTCAGATCGGGCTGTGTC
Internal bar code:GTGCACGTGATCCCTCGACGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:336
LEAP-Seq percent confirming:99.4641
LEAP-Seq n confirming:928
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCCCTCTCTGTCCATGT
Suggested primer 2:GGTTGTAAGTGCAGGACGGT