| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.099123 |
| Chromosome: | chromosome_2 |
| Location: | 80283 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g073750 | KIN9-2,KLP1 | Kinesin motor protein, kinesin-9 (Kif9) family | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTCCGCCGCCTGCCTTACAGAGCTCTGAC |
| Internal bar code: | ACGATGGGCCGGTTTCGCCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.7785 |
| LEAP-Seq n confirming: | 9460 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAAACAACCCTGCTCAC |
| Suggested primer 2: | GTCACAAAAGAGCGCAATCA |