| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099144 |
| Chromosome: | chromosome 1 |
| Location: | 5270824 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036800 | KIN9-1,KIN9A | Kinesin motor protein; (1 of 1) PTHR24115//PTHR24115:SF194 - FAMILY NOT NAMED // KINESIN-LIKE PROTEIN KIF6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGCCCGCCACTGCCTCGTGTACAAGCC |
| Internal bar code: | TAGCGCTTGGCTCGAGCCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 98.1711 |
| LEAP-Seq n confirming: | 9179 |
| LEAP-Seq n nonconfirming: | 171 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGCGCTCATATCACAGG |
| Suggested primer 2: | ATGCTGGTAAAAGGTGGTGC |