| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.099149 |
| Chromosome: | chromosome 14 |
| Location: | 3278138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629650 | NIK1 | Nickel transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAATGCCGATGTGGCGGCAGCGCTGAA |
| Internal bar code: | GCCGCGTCTATGCGTCAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 519 |
| LEAP-Seq percent confirming: | 97.8941 |
| LEAP-Seq n confirming: | 4602 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCACATTGGGATTTTGAG |
| Suggested primer 2: | GTTAGGCAGCGTAGACAGGC |