| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099149 |
| Chromosome: | chromosome 14 |
| Location: | 3278152 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629650 | NIK1 | Nickel transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGACGGTAGTCCCGACATACCTTGCAAA |
| Internal bar code: | ACAGTCGATCATCGCGGCCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 8.05991 |
| LEAP-Seq n confirming: | 113 |
| LEAP-Seq n nonconfirming: | 1289 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGGCAGCGTAGACAGGC |
| Suggested primer 2: | AGGCACATTGGGATTTTGAG |