| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.099156 |
| Chromosome: | chromosome 16 |
| Location: | 3753876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689250 | GTR23 | (1 of 1) PF04564//PF06701 - U-box domain (U-box) // Mib_herc2 (MIB_HERC2); Putative ubiquitin-protein ligase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCGGTTGAAAATGTCGAGCTTACGTTG |
| Internal bar code: | GCGGGGTCCCCACGCTTGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 99.6089 |
| LEAP-Seq n confirming: | 2547 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACATGCGGTGTCCATGACT |
| Suggested primer 2: | TCTGATGGCAGTTTGTCAGC |